Non-transferrin-bound iron assay system utilizing a conventional automated analyzer
نویسندگان
چکیده
منابع مشابه
Bleomycin-detectable iron assay for non-transferrin-bound iron in hematologic malignancies.
BACKGROUND A microwell modification of the bleomycin assay for determining non-transferrin-bound iron (NTBI) was evaluated and compared with a chelation method. METHODS The bleomycin assay reagent and sample volumes were halved, and measurements were done in microwell plates. Samples from patients treated for hematologic malignancies were studied. The chelation method was based on mobilizatio...
متن کاملTransferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron.
BACKGROUND/AIMS Transferrin receptor 2 appears to have dual roles in iron metabolism; one is signalling, the other is iron transport. It is sensitive to high levels of diferric transferrin, which is associated with disorders of iron overload. Also present in these disorders are increased levels of plasma non-transferrin-bound iron. This study sought to clarify the role of transferrin receptor 2...
متن کاملClinical and methodological factors affecting non-transferrin-bound iron values using a novel fluorescent bead assay
Nontransferrin-bound iron (NTBI) is a heterogeneously speciated plasma iron, typically detectable when transferrin saturation (TfSat) exceeds 75%. Here, we examine factors affecting NTBI levels by a recently discovered direct chelator-based (CP851) fluorescent bead-linked flow-cytometric assay (bead-NTBI), compared with the established indirect nitrilotriacetate (NTA) assay in 122 iron-overload...
متن کاملNon-transferrin bound iron: a key role in iron overload and iron toxicity.
BACKGROUND Besides transferrin iron, which represents the normal form of circulating iron, non-transferrin bound iron (NTBI) has been identified in the plasma of patients with various pathological conditions in which transferrin saturation is significantly elevated. SCOPE OF THE REVIEW To show that: i) NTBI is present not only during chronic iron overload disorders (hemochromatosis, transfusi...
متن کاملNon-transferrin bound iron and neutropenia after cytotoxic chemotherapy.
AIMS To investigate and characterise the appearance of non-transferrin bound iron (NTBI) in the serum of patients after cytotoxic chemotherapy and to compare this with the onset and duration of neutropenia. METHOD Non-transferrin bound iron was measured by a bleomycin assay in patients undergoing intensive chemotherapy for treatment of acute leukaemia or lymphoma. RESULTS NTBI was detected ...
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ژورنال
عنوان ژورنال: Clinica Chimica Acta
سال: 2014
ISSN: 0009-8981
DOI: 10.1016/j.cca.2014.07.013